Why plasmid map

Then a cut is made each. P are repeated i. Thus you have a plasmid map. About Me. I studied Biochemistry at the University of Lagos, Nigeria. As my first degree as a graduate, I plan to advance my career in the near future in the Biotechnology field. I love creativity and learning new things, and want to be a part of an evolution in life sciences. Please feel free to reach out via twitter ibmathiass. Reblogged this on Long Road to Innovation and commented: Learn the basics of plasmid mapping!

Like Like. You are commenting using your WordPress. You are commenting using your Google account. You are commenting using your Twitter account. You are commenting using your Facebook account. Notify me of new comments via email. Notify me of new posts via email. This video shows how to use SeqBuilder Pro to create a sequence from scratch or edit an existing sequence.

This video also shows how to search within a sequence for ORFs, features and more, how to change the window layout, and how to add a comment to a sequence file. This video demonstrates the plasmid auto-annotation functionality in SeqBuilder Pro which allows you to accurately and automatically annotate your sequences using a carefully curated database of features.

Simply select your sequences and SeqBuilder Pro will provide you with a list of matched features for your consideration. This video shows you how to rearrange and edit the annotations that are displayed on your plasmid map to prepare it for publication. The Enzymes panel lets you control which enzymes are displayed on your plasmid map. You can choose to apply groups of enzymes based on the frequency of cuts, type or class, site complexity, overhang compatibility…. You can choose to apply groups of enzymes based on the frequency of cuts, type or class, site complexity, overhang compatibility, or an intersection of any of these groups.

To move a restriction enzyme label on your plasmid map, first click on the label to select it. Then, click and drag the label to the desired location. Other enzyme labels on your plasmid map will be moved automatically to avoid overlapping. Here, two portions of the DNA sample are individually digested with different restriction enzymes, and a third portion of the DNA sample is double-digested with both restriction enzymes at the same time.

Next, each digestion sample is separated using gel electrophoresis, and the sizes of the DNA fragments are recorded. The total length of the fragments in each digestion will be equal. However, because the length of each individual DNA fragment depends upon the positions of its restriction sites, each restriction site can be mapped according to the lengths of the fragments.

The information from the double-digestion is particularly useful for correctly mapping the sites. The final drawing of the DNA segment that shows the positions of the restriction sites is called a restriction map.